Conduct advanced molecular biology and stem cell research in an on‑site postdoctoral capacity within the Duke University Department of Obstetrics and Gynecology.
Responsibilities
Perform 2D and 3D stem cell culture, including human peripheral blood mononuclear cell isolation
Prepare samples for immunofluorescence staining and imaging with light, fluorescence and confocal microscopes
Conduct western blot and ELISA assays
Design and execute molecular assays such as DNA/RNA extraction, PCR, qPCR
Generate single‑cell and single‑nuclei RNA sequencing libraries, ChIP‑seq and ATAC‑seq
Requirements
Ph.D. or equivalent doctorate (e.g., Sc.D., M.D., D.V.M.)
Design, perform, and analyze molecular biology experiments, including quantitative PCR (qPCR), droplet digital PCR (ddPCR), gel electrophoresis, and Western blotting to evaluate gene and protein expression.
Conduct protein-based assays and proteomic studies, including immunoprecipitation (IP) and protein quantification using HTRF, AlphaLISA, and MSD platforms.
Perform routine mammalian cell culture, plasmid transfection, and viral transduction using human iPSC-derived neurons and established cell lines.
Support target validation studies through immunostaining, fluorescence microscopy, and quantitative image analysis.
Conduct co-localization, morphological, and phenotypic assessments to characterize candidate therapeutic targets relevant to ALS and other neurodegenerative diseases.
Postdoctoral AssociateNewDuke University · Durham, US