- Bothell, Washington, United States
- On-site
- Full-time · Entry
- $90K–$115K per year
- 0–3 years
Drive design, expression, and purification of recombinant antigens to support antibody discovery and engineering programs.
Compensation
The expected base salary range for this position is $90,000 - $115,000 annually. Actual compensation will be based on a variety of factors, including but not limited to a candidate’s level qualifications, experience, and skills.
Responsibilities
- Design and clone antigen constructs using molecular biology techniques, including vector design, cloning strategies, and sequence verification.
- Develop and optimize transient and stable mammalian expression systems (e.g., HEK293 or CHO) to produce secreted and cell-surface antigens.
- Establish and execute purification workflows for recombinant proteins, including affinity, ion-exchange, and size-exclusion chromatography.
- Troubleshoot expression and purification problems, adjusting constructs, culture conditions, and purification strategies as needed.
- Establish and execute quality control for each antigen batch, including purity, identity, and homogeneity.
Requirements
- PhD in Biochemistry
- PhD in Molecular Biology
- PhD in related field
- Nice to have: antibody discovery
- Nice to have: immunization