.jpg?1741901472){kind=icon}
- Emeryville, California, United States
- On-site
- Full-time · Mid
- $155K–$160K per year
- 3+ years
Profluent is hiring a Scientist II to build and own their yeast surface display platform for binder generation, producing data to train and evaluate protein design models.
Compensation
$155,000—$160,000 USD
Responsibilities
- Establish and validate a yeast surface display platform for binder generation, including display vector and strain selection, library design and construction, and FACS-based selection and sorting
- Design and execute yeast display campaigns across binder formats (e.g., de novo miniproteins, VHHs, scFvs, and alternative scaffolds) against a range of targets
- Take workflows from concept to a robust, repeatable process: define controls, QC, and acceptance criteria, and characterize reproducibility, signal-to-noise, and the relationship between sort-bin enrichment and binding strength
- Build NGS readouts into selections and deep mutational scanning workflows, converting raw sequencing counts into quantitative binding metrics that ML models can learn from
- Partner with the ML scientists, and Protein Design scientists to scope campaigns (targets, library composition, readouts) and to define what 'model-ready' binding data looks like
Requirements
- PhD in Molecular Biology
- Biochemistry
- Protein Engineering
- Biophysics
- Immunology