Profluent is hiring a Scientist II to build and own their yeast surface display platform for binder generation, producing data to train and evaluate protein design models.
Compensation
$155,000—$160,000 USD
Responsibilities
Establish and validate a yeast surface display platform for binder generation, including display vector and strain selection, library design and construction, and FACS-based selection and sorting
Design and execute yeast display campaigns across binder formats (e.g., de novo miniproteins, VHHs, scFvs, and alternative scaffolds) against a range of targets
Take workflows from concept to a robust, repeatable process: define controls, QC, and acceptance criteria, and characterize reproducibility, signal-to-noise, and the relationship between sort-bin enrichment and binding strength
Build NGS readouts into selections and deep mutational scanning workflows, converting raw sequencing counts into quantitative binding metrics that ML models can learn from
Partner with the ML scientists, and Protein Design scientists to scope campaigns (targets, library composition, readouts) and to define what 'model-ready' binding data looks like
Requirements
PhD in Molecular Biology
Biochemistry
Protein Engineering
Biophysics
Immunology
Skills
Research Associate, Applied Cell Line Development & Customer Programs
Assist in mammalian cell line development and protein production, generating and characterizing cell lines for internal research and customer programs.
Responsibilities
Own and drive workflows using mammalian cell culture, primarily working with suspension HEK and suspension CHO
Generate stable cell lines via transfection (ie electroporation) and selection and serve as the primary technical owner of many genetically distinct cell lines
Handle protein production workflows at different scales - deep well plates and shake flasks, potentially including small scale bioreactors such as the Ambr system
Execute downstream readouts such as cell counting, metabolite measurements, Octet BLI, flow cytometry
Perform single cell isolation using methods such as FACS, cell deposition, or Beacon
Requirements
Bachelor's degree in Biology, Bioengineering, Biotechnology, or a related field
Lead scientist responsible for analytical and functional characterization of biopharmaceutical products, including monoclonal antibodies and antibody-like biologics.
Responsibilities
Develop and execute SPR assays to generate selection criteria for antibody panels
Evaluate and integrate protein–protein interaction technologies to support therapeutic candidate selection
Implement high-throughput epitope binning for large antibody panels
Perform antibody Fc characterization studies for regulatory submissions
Solve technical problems and develop new approaches to advance characterization and screening technologies
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