Senior Scientist to serve as the protein purification subject-matter expert within the Biochemistry and Reagents R&D group.
Compensation
$128,800.00 - $193,200.00
Responsibilities
Build, run, and continuously improve purification pipelines for nucleic acid–modifying enzymes at multiple research scales.
Serve as the group's subject-matter expert on protein expression and purification; advise colleagues on construct design, expression host, affinity tags, and purification strategy.
Develop expression and purification strategies for challenging targets, including poorly soluble proteins, multi-subunit complexes, and novel homologs, where standard protocols fall short; evaluate alternative strategies side by side to maximize yield, activity, and purity.
Design and execute high-throughput 24- to 96-plex expression and purification campaigns on lab automation platforms to screen enzyme variants and homologs in parallel.
Perform targeted individual purifications on ÄKTA FPLC systems using affinity, ion-exchange, size-exclusion, and other chromatography methods.
Requirements
PhD in Biochemistry, Molecular Biology, Biophysics, Chemistry, Bioengineering, or a related field
Nice to have: industry R&D experience in biotechnology, life science tools, or enzyme and reagent development
Nice to have: single-molecule biochemistry or biophysics
Nice to have: DNA sequencing, especially NGS library preparation or long-read sequencing
Nice to have: biochemical assays for polymerases, ligases, nucleases, and/or Cas proteins
Profluent is hiring a Scientist II to build and own their yeast surface display platform for binder generation, producing data to train and evaluate protein design models.
Compensation
$155,000—$160,000 USD
Responsibilities
Establish and validate a yeast surface display platform for binder generation, including display vector and strain selection, library design and construction, and FACS-based selection and sorting
Design and execute yeast display campaigns across binder formats (e.g., de novo miniproteins, VHHs, scFvs, and alternative scaffolds) against a range of targets
Take workflows from concept to a robust, repeatable process: define controls, QC, and acceptance criteria, and characterize reproducibility, signal-to-noise, and the relationship between sort-bin enrichment and binding strength
Build NGS readouts into selections and deep mutational scanning workflows, converting raw sequencing counts into quantitative binding metrics that ML models can learn from
Partner with the ML scientists, and Protein Design scientists to scope campaigns (targets, library composition, readouts) and to define what 'model-ready' binding data looks like
Perform end-to-end generation of omics data using high-throughput laboratory automation. Execute automated DNA and RNA workflows, including PCR reactions and sample processing. Operate, program, and troubleshoot automated liquid-handling platforms to support genomics workflows. Perform wet chemistry procedures. Support Next Generation Sequencing (NGS) workflows using Illumina platforms.
Compensation
Pay Rate: $72.62/hr.
Responsibilities
Perform end-to-end generation of omics data using high-throughput laboratory automation.
Execute automated DNA and RNA workflows, including PCR reactions and sample processing.
Operate, program, and troubleshoot automated liquid-handling platforms to support genomics workflows.
Perform wet chemistry procedures, including pipetting, buffer preparation, filtration, dilution, and stoichiometric calculations.
Support Next Generation Sequencing (NGS) workflows using Illumina platforms.
Requirements
Bachelor's degree
Master's degree
PhD
Nice to have: Analytical instrumentation: Plate readers, thermocyclers
Perform laboratory testing and testing using automated analyzers, microscopes, and diagnostic tools to diagnose animal illnesses, injuries, and diseases in support of veterinary treatment.
Compensation
Hourly rates targeting: $30 / hr (will be skillset based)... Opportunity for annual cash bonus and merit pay increase consideration
Responsibilities
Perform diagnostic tests on blood, urine, fecal or pet biopsy samples
Set up and run lab tests
Read and release results according to SOPs
Use automated analyzers, microscopes, and other laboratory diagnostic tools
Perform maintenance, daily start up, and troubleshooting on analyzers
Requirements
Associate's or Bachelor's degree in Medical Laboratory Science (MLS)
Clinical Laboratory Science (CLS)
Medical Technology (MT)
Medical Laboratory Technology (MLT)
related biological, biomedical, or chemical science field
Perform laboratory testing to diagnose animal illnesses, injuries and diseases in a high-volume veterinary diagnostic environment.
Compensation
Hourly rates targeting: $30 / hr (will be skillset based)
Responsibilities
Use automated analyzers, microscopes and other laboratory diagnostic tools to perform analyses and tests
Perform diagnostic tests on blood, urine, fecal or pet biopsy samples
Set up and run lab tests, read and release results according to SOPs
Specialize in an assigned department
Maintain and troubleshoot analyzers
Requirements
Associate's or Bachelor's degree in Medical Laboratory Science (MLS), Clinical Laboratory Science (CLS), Medical Technology (MT), Medical Laboratory Technology (MLT), or related biological, biomedical, or chemical science field
Perform laboratory testing to help veterinarians diagnose and treat pets using automated analyzers, microscopes, and other diagnostic tools.
Compensation
Hourly rates targeting: $30 / hr (will be skillset based). Opportunity for annual cash bonus and merit pay increase consideration
Responsibilities
Use automated analyzers, microscopes and other laboratory diagnostic tools to perform analyses and tests that diagnose animal illnesses, injuries and diseases
Perform diagnostic tests on blood, urine, fecal or pet biopsy samples
Set up and run lab tests, then read and release results according to SOPs
Specialize in assigned departments such as Hematology, Chemistry, Microbiology, Immunology/Serology, Urinalysis and Cytology
Perform maintenance, daily start up and troubleshooting on analyzers like Beckman, Sysmex, Novus, Immulite, Hamilton, Cellavision
Requirements
Associate's or Bachelor's degree in Medical Laboratory Science (MLS), Clinical Laboratory Science (CLS), Medical Technology (MT), Medical Laboratory Technology (MLT), or a related biological, biomedical, or chemical science field
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