Scientist or engineer focused on expansion microscopy development and protocol optimization within a sample production pipeline.
Responsibilities
Independently design, coordinate, and execute bench level experiments focused on expansion microscopy.
Operate within our sample production pipeline to ensure a continuous flow of high-quality gels.
Iteratively optimize expansion microscopy workflows by designing, sourcing and adapting techniques and methodologies from the literature and scientific community.
Pilot variations of expansion microscopy protocols focused on optimizing signal retention for multi-round cycles of immunostaining, imaging, and signal extinction.
Pilot methods for increasing diffusion within expanded hydrogel/tissue hybrids.
Requirements
Ph.D. in biology, chemistry, bioengineering, materials science, mechanical engineering, or equivalent research or industry experience
Nice to have: advanced optical microscope modalities (spinning-disc confocal or light-sheet microscopy)
Nice to have: quantitative scoring systems for expansion microscopy or imaging data
Nice to have: scaling expansion microscopy throughput in routine production environments
Nice to have: automating lab workflows (liquid handlers, microcontrollers like Arduino or Raspberry Pi, scripting in Python)
Senior Scientist, AI Pathologist (w/f/d) – fixed‑term maternity cover at AstraZeneca’s Computational Pathology site in Munich, Germany.
Responsibilities
Provide pathology expertise for AI and biomarker projects, translating disease biology, tissue morphology and diagnostic criteria into requirements for datasets, annotations and model evaluation.
Support biomarker discovery and study design, including tissue and image requirements, cohort and sample selection, staining characteristics and relevant pathology subgroups.
Develop and review annotation and ground‑truth strategies for AI model development, including annotation criteria and pathology review of annotated datasets.
Evaluate AI model performance from a pathology perspective, identifying relevant patterns, potential failure modes and limitations across tissues, morphology, staining and patient subgroups.
Ensure pathology and image‑analysis quality, including the biological and clinical interpretation and explainability of model outputs.
Requirements
Medical degree with formal training and board certification in anatomic/anatomical pathology, or an equivalent recognised qualification, with strong expertise in oncology, histopathology and immunohistochemistry (IHC).
Veterinary degree may also be considered for candidates with highly relevant experience in human tissue and disease biology.
Nice to have: Experience with quantitative image analysis and advanced or multiplex imaging, such as multiplex immunofluorescence or multiplex IHC.
Support the development and expansion of spatial biology capabilities for preclinical and translational biomarker discovery in the Oncology Research department.
Compensation
Pay: $38.93/Hour
Responsibilities
Develop, optimize, and execute high-plex tissue-based assays, including multiplex immunofluorescence and other spatial protein profiling approaches in human and mouse tissues.
Design and optimize multiplex antibody panels, including antibody selection, staining conditions, controls, panel compatibility, tissue-specific optimization, and assay performance.
Support the establishment and application of spatial transcriptomics and emerging spatial profiling technologies, including assay and data QC.
Develop integrated workflows spanning tissue preparation, staining, imaging, spatial profiling, and quantitative analysis, troubleshooting assays to improve robustness, reproducibility, and data quality.
Support acquisition and analysis of whole-slide, multiplex, and spatial imaging datasets, including tissue annotation, cell segmentation, phenotyping, spatial quantification, and visualization.
Requirements
Bachelor’s or Master’s degree in Biology, Histotechnology, Pathology, Biomedical Sciences, or a related discipline
Support the development and expansion of spatial biology capabilities for preclinical and translational biomarker discovery.
Compensation
Pay Rate Range: $38.66 - $41.42/hr. on W2.
Responsibilities
Develop, optimize, and execute high-plex tissue-based assays, including multiplex immunofluorescence and other spatial protein profiling approaches in human and mouse tissues.
Design and optimize multiplex antibody panels, including antibody selection, staining conditions, controls, panel compatibility, tissue-specific optimization, and assay performance.
Support the establishment and application of spatial transcriptomics and emerging spatial profiling technologies, including assay and data QC.
Develop integrated workflows spanning tissue preparation, staining, imaging, spatial profiling, and quantitative analysis, troubleshooting assays to improve robustness, reproducibility, and data quality.
Support acquisition and analysis of whole-slide, multiplex, and spatial imaging datasets, including tissue annotation, cell segmentation, phenotyping, spatial quantification, and visualization.
Requirements
Bachelor's or Master's degree in Biology, Histotechnology, Pathology, Biomedical Sciences, or a related discipline.
Nice to have: High-plex/multiplex protein imaging
Nice to have: Spatial transcriptomics or related spatial gene-expression technologies
Support exploratory discovery research focused on the molecular and cellular mechanisms underlying ALS within the Neurology Research Unit, ALS Research Group.
Responsibilities
Design, execute, and analyze experiments investigating genomic and proteomic alterations in ALS-relevant pathways
Perform molecular biology assays such as qPCR, ddPCR, gel electrophoresis, western blotting
Conduct proteomic studies including IP, HTRF, AlphaLISA, MSD assay platforms
Maintain mammalian cell culture, plasmid transfection, viral transduction of iPSC-derived neurons and cell lines
Support target validation studies through immunostaining, fluorescence microscopy, and quantitative image analysis
Requirements
PhD or MS in Biochemistry, Molecular Biology, Neuroscience or related discipline
Design, perform, and analyze molecular biology experiments, including quantitative PCR (qPCR), droplet digital PCR (ddPCR), gel electrophoresis, and Western blotting to evaluate gene and protein expression.
Conduct protein-based assays and proteomic studies, including immunoprecipitation (IP) and protein quantification using HTRF, AlphaLISA, and MSD platforms.
Perform routine mammalian cell culture, plasmid transfection, and viral transduction using human iPSC-derived neurons and established cell lines.
Support target validation studies through immunostaining, fluorescence microscopy, and quantitative image analysis.
Conduct co-localization, morphological, and phenotypic assessments to characterize candidate therapeutic targets relevant to ALS and other neurodegenerative diseases.
Support discovery-stage ALS research by executing molecular and cell-based experiments that contribute to target identification and validation efforts.
Responsibilities
Design, execute, and analyze experiments investigating genomic and proteomic alterations in ALS-relevant pathways
Perform molecular biology assays such as qPCR, ddPCR, and gel electrophoresis to assess gene expression
Conduct western blot analyses to assess protein expression
Perform proteomic studies including immunoprecipitation and protein quantification using HTRF, AlphaLISA, and MSD assay platforms
Maintain routine mammalian cell culture; plasmid transfection and viral transduction of iPSC-derived neurons and established cell lines
Senior Scientist, AI Pathologist (w/f/d)AstraZeneca · Munich, Germany
Post Doctoral Associate-MinhasUniversity of Pittsburgh · Pittsburgh, US